The Polymerase Chain Reaction

The Polymerase Chain Reaction

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James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. qIt has not escaped our notice, q Francis wrote, qthat the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material.q By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act q. . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one beforeq and, moreover, q ...Therefore, a wide variety of AMP-FLP loci can be accommodated with one general analytical system. Sample loading ... This automated approach can provide some conveniences compared with the more routine manual approach. An internalanbsp;...


Title:The Polymerase Chain Reaction
Author: Kary B. Mullis, Francois Ferre, Richard A. Gibbs
Publisher:Springer Science & Business Media - 2012-02-02
ISBN-13:

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